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KMID : 0352720180420040504
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Journal of Ginseng Research
2018 Volume.42 No. 4 p.504 ~ p.511
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Highly regioselective biotransformation of ginsenoside Rb2 into compound Y and compound K by b-glycosidase purified from Armillaria mellea mycelia
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Kim Min-Ji
Upadhyaya Jitendra
Yoon Min-Sun
Ryu Nam-Soo
Song Young-Eun
Park Hee-Won
Kim Young-Hoi
Kim Myung-Kon
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Abstract
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Background: The biological activities of ginseng saponins (ginsenosides) are associated with type, number, and position of sugar moieties linked to aglycone skeletons. Deglycosylated minor ginsenosides are known to be more biologically active than major ginsenosides. Accordingly, the deglycosylation of major ginsenosides can provide the multibioactive effects of ginsenosides. The purpose of this study was to transform ginsenoside Rb2, one of the protopanaxadiol-type major ginsenosides, into minor ginsenosides using b-glycosidase (BG-1) purified from Armillaria mellea mycelium.
Methods: Ginsenoside Rb2 was hydrolyzed by using BG-1; the hydrolytic properties of Rb2 by BG-1 were also characterized. In addition, the influence of reaction conditions such as reaction time, pH, and temperature, and transformation pathways of Rb2, Rd, F2, compound O (C-O), and C-Y by treatment with BG-1 were investigated.
Results: BG-1 first hydrolyzes 3-O-outer b-D-glucoside of Rb2, then 3-O-b-D-glucoside of C-O into C-Y. C-Y was gradually converted into C-K with a prolonged reaction time, but the pathway of Rb2/Rd/F2/ C-K was not observed. The optimum reaction conditions for C-Y and C-K formation from Rb2 by BG-1 were pH 4.0e4.5, temperature 45e60C, and reaction time 72e96 h.
Conclusion: b-Glycosidase purified from A. mellea mycelium can be efficiently used to transform Rb2 into C-Y and C-K. To our best knowledge, this is the first result of transformation from Rb2 into C-Y and C-K by basidiomycete mushroom enzyme.
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KEYWORD
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Armillaria mellea, b-glycosidase, biotransformation, compound Y, ginsenoside Rb2
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